Composite

Part:BBa_K2112001:Design

Designed by: Fanglin Zhao   Group: iGEM16_LN-Shiyan-China   (2016-10-14)


OP Pesticides Residue Killer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 994
    Illegal XhoI site found at 2095
    Illegal XhoI site found at 3196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 1192
    Illegal NgoMIV site found at 2293
    Illegal AgeI site found at 286
    Illegal AgeI site found at 625
    Illegal AgeI site found at 1387
    Illegal AgeI site found at 1726
    Illegal AgeI site found at 2488
    Illegal AgeI site found at 2827
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3630
    Illegal BsaI site found at 3936


Design Notes

The his-tag can allow us to purify the protein after the bacteria secrete it, so that if the project can be use in real life, the posibility of risk can be limited by giving the users the purified protein to degrade organophosphorous instead of using the bacteria.


Source

The ccdB gene is found by other teams, we use it in our part to achieve the goal.

References